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  Projets de mise en oeuv...  
Détermination des doses minimales biologiquement efficaces d'herbicides pour le désherbage dans la rotation maïs-soya
Determining the minimum biologically effective dose of herbicides for weed control in corn-soybean rotations
  Categorization and nume...  
Nous analysons l’effet de l’insecticide pyréthroïde bifenthrine incorporé au sol à raison de 100, 200 et 300 g m.a./ha sur les larves du dernier stade du taupin obscur (Agriotes obscurus) (Coleoptera: Elateridae), et nous présentons une méthode permettant d’évaluer l’état de santé et la mobilité des larves en fonction du temps. Les larves de taupin sont devenues moribondes dans l’heure qui a suivi leur transfert dans du sol amendé avec de la bifenthrine à l’une ou l’autre des trois doses susmentionnées.
We discuss the effect of the pyrethroid insecticide bifenthrin incorporated into soil at 100, 200, and 300 g AI/ha on late-instar larvae of the dusky wireworm, Agriotes obscurus (Coleoptera: Elateridae), and present a method of assessing wireworm health and mobility over time. Wireworms became moribund within 1 h of placement in soil amended with bifenthrin at all rates. After 2 weeks of morbidity in amended soils, wireworms recovered within 7 days of being placed in clean soil. A considerable proportion (0.13–0.93) of wireworms placed in amended soil moved to the soil surface and remained there for 2 weeks or more in a moribund state; wireworms transferred to clean soil no longer moved to the soil surface. Eight distinct mobility categories were observed and are described, and a new method for assessing wireworm health and mobility over time is discussed.
  Suitable dose of  
Nous avons irradié des graines de six obtentions de trois espèces de Roegneria avec le rayonnement gamma du 60Co, à différentes doses (50, 100, 150, 200, 250, 300 et 400 Gy). Suite à ces traitements, nous avons étudié l’énergie de germination, la vitesse de germination, la hauteur des semis, la hauteur des plants, le taux de survie des plants et la grenaison.
Seeds from six accessions of three species of Roegneria were radiated with 60Co γ-ray at different doses (50, 100, 150, 200, 250, 300 and 400 Gy). Following these treatments, germination energy, germination rate, seedling height, plant height, plant survival, and seed set were observed. Plant survival was highly correlated with seedling height (R 2 > 0.91, P < 0.01) and seed set (R 2 > 0.82, P < 0.01). The semi-lethal dose of each accession, calculated using a ‘Multi-target single-hit’ model, ranged from 60 to 173 Gy. The most suitable absorbed doses for each accession were deduced from these data. The suitable doses for Roegneria kamoji, Roegneria ciliaris and Roegneria japonensis were 65–100 Gy, 63–150 Gy and 80–170 Gy, respectively. According to the range of suitable doses, R. kamoji (Pr87-88-353) was the most sensitive to radiation, and R. japonensis (88-89-267) was the most resistant to radiation. Suitable doses of R. ciliaris were close to that of R. kamoji (ZY1007). This research provides preliminary guidelines for radiation induced mutagenesis in Roegneria.
  Suitable dose of  
Nous avons irradié des graines de six obtentions de trois espèces de Roegneria avec le rayonnement gamma du 60Co, à différentes doses (50, 100, 150, 200, 250, 300 et 400 Gy). Suite à ces traitements, nous avons étudié l’énergie de germination, la vitesse de germination, la hauteur des semis, la hauteur des plants, le taux de survie des plants et la grenaison.
Seeds from six accessions of three species of Roegneria were radiated with 60Co γ-ray at different doses (50, 100, 150, 200, 250, 300 and 400 Gy). Following these treatments, germination energy, germination rate, seedling height, plant height, plant survival, and seed set were observed. Plant survival was highly correlated with seedling height (R 2 > 0.91, P < 0.01) and seed set (R 2 > 0.82, P < 0.01). The semi-lethal dose of each accession, calculated using a ‘Multi-target single-hit’ model, ranged from 60 to 173 Gy. The most suitable absorbed doses for each accession were deduced from these data. The suitable doses for Roegneria kamoji, Roegneria ciliaris and Roegneria japonensis were 65–100 Gy, 63–150 Gy and 80–170 Gy, respectively. According to the range of suitable doses, R. kamoji (Pr87-88-353) was the most sensitive to radiation, and R. japonensis (88-89-267) was the most resistant to radiation. Suitable doses of R. ciliaris were close to that of R. kamoji (ZY1007). This research provides preliminary guidelines for radiation induced mutagenesis in Roegneria.
  Acetolactate Synthase (...  
Dans le cadre d’un criblage réalisé en serre, toutes les descendances F1 étudiées se sont révélées résistantes à deux herbicides à base d’inhibiteurs de l’ALS, soit le thifensulfuron/tribénuron, du groupe des sulfonylurées, et le florasulam, du groupe des triazolopyrimidines; un essai de réaction des pousses à diverses doses a révélé que cette population était 10 fois moins sensible au thifensulfuron/tribénuron et 20 fois moins sensible au florasulam que la population sensible utilisée comme témoin.
Wild buckwheat is the most abundant broadleaf weed across the Prairie region of western Canada. Acetolactate synthase (ALS)-inhibiting herbicides are commonly used to control this species and other broadleaf weeds in cereal crops. A field survey in Alberta in 2007 identified a single population that was putatively resistant to ALS-inhibiting herbicides. In herbicide resistance screening in the greenhouse, all F1 progeny tested were resistant to the ALS-inhibiting herbicides thifensulfuron/tribenuron, a sulfonylurea herbicide, or florasulam, a triazolopyrimidine herbicide; dose response of shoot biomass indicated the population was 10- and 20-fold less sensitive to thifensulfuron/tribenuron and florasulam, respectively, than a susceptible control population. ALS gene sequencing of 24 F1 progeny indicated that the Trp574Leu target-site mutation was responsible for conferring ALS-inhibitor resistance in this biotype, the first global report of ALSinhibitor resistance for this species. Because this mutation typically endows high-level resistance across all five ALSinhibitor classes, this wild buckwheat biotype may only be controlled by a different site-of-action herbicide.
  Suitable dose of  
Nous avons irradié des graines de six obtentions de trois espèces de Roegneria avec le rayonnement gamma du 60Co, à différentes doses (50, 100, 150, 200, 250, 300 et 400 Gy). Suite à ces traitements, nous avons étudié l’énergie de germination, la vitesse de germination, la hauteur des semis, la hauteur des plants, le taux de survie des plants et la grenaison.
Seeds from six accessions of three species of Roegneria were radiated with 60Co γ-ray at different doses (50, 100, 150, 200, 250, 300 and 400 Gy). Following these treatments, germination energy, germination rate, seedling height, plant height, plant survival, and seed set were observed. Plant survival was highly correlated with seedling height (R 2 > 0.91, P < 0.01) and seed set (R 2 > 0.82, P < 0.01). The semi-lethal dose of each accession, calculated using a ‘Multi-target single-hit’ model, ranged from 60 to 173 Gy. The most suitable absorbed doses for each accession were deduced from these data. The suitable doses for Roegneria kamoji, Roegneria ciliaris and Roegneria japonensis were 65–100 Gy, 63–150 Gy and 80–170 Gy, respectively. According to the range of suitable doses, R. kamoji (Pr87-88-353) was the most sensitive to radiation, and R. japonensis (88-89-267) was the most resistant to radiation. Suitable doses of R. ciliaris were close to that of R. kamoji (ZY1007). This research provides preliminary guidelines for radiation induced mutagenesis in Roegneria.
  Réaction aux engrais N ...  
Les relations indiquent que le canola hybride a un rendement grainier supérieur à celui du canola à pollinisation libre, indifféremment de l’apport d’engrais azoté, et que les doses optimales d’engrais azoté sont d’environ 50 % plus élevées pour le canola hybride que pour le canola à pollinisation libre.
Canola is a viable crop when grown under fallow in the semiarid prairie, but is also grown in longer rotations, most often no-till seeded into standing stubble. Selecting the proper N fertilizer rate is a very challenging production decision, but most of the available nitrogen response for canola has been derived for the more subhumid parts of the Canadian prairies. We developed simple quadratic equations to describe the yield relationship for stubble-seeded open-pollinated and hybrid canola in the semiarid Canadian prairie. These relationships indicate that hybrid canola produced higher grain yields at all fertilizer rates and had optimum N fertilizer rates about 50% higher than those for open-pollinated canola.
  Suitable dose of  
Nous avons irradié des graines de six obtentions de trois espèces de Roegneria avec le rayonnement gamma du 60Co, à différentes doses (50, 100, 150, 200, 250, 300 et 400 Gy). Suite à ces traitements, nous avons étudié l’énergie de germination, la vitesse de germination, la hauteur des semis, la hauteur des plants, le taux de survie des plants et la grenaison.
Seeds from six accessions of three species of Roegneria were radiated with 60Co γ-ray at different doses (50, 100, 150, 200, 250, 300 and 400 Gy). Following these treatments, germination energy, germination rate, seedling height, plant height, plant survival, and seed set were observed. Plant survival was highly correlated with seedling height (R 2 > 0.91, P < 0.01) and seed set (R 2 > 0.82, P < 0.01). The semi-lethal dose of each accession, calculated using a ‘Multi-target single-hit’ model, ranged from 60 to 173 Gy. The most suitable absorbed doses for each accession were deduced from these data. The suitable doses for Roegneria kamoji, Roegneria ciliaris and Roegneria japonensis were 65–100 Gy, 63–150 Gy and 80–170 Gy, respectively. According to the range of suitable doses, R. kamoji (Pr87-88-353) was the most sensitive to radiation, and R. japonensis (88-89-267) was the most resistant to radiation. Suitable doses of R. ciliaris were close to that of R. kamoji (ZY1007). This research provides preliminary guidelines for radiation induced mutagenesis in Roegneria.
  Long-Term Cattle Manure...  
Les épandages de fumier à long terme ont accru les teneurs du sol en matière organique, en azote, en phosphore, en sel et en minéraux traces. Le rendement en paille a augmenté à toutes les doses de fumier, mais les plus fortes doses ont réduit le rendement en grains et nuit à la qualité de la culture.
A field experiment was started in 1973 to determine the safe loading capacity of soil with cattle feedlot manure. Manure was applied annually at 0, 30, 60, and 90 tonne ha-1 (wet weight) under rainfed and 0, 60, 120, and 180 tonne ha-1 under irrigated conditions. The long-term manure applications resulted in increased levels of organic matter, N, P, salt and trace minerals in soil. This increased straw yield at all manure rates, but reduced grain yields at higher rates and affected crop quality. Increased nutrient levels also increase the potential for nutrient losses and surface and groundwater contamination. The soil enrichments were long-lasting and could pose environmental threats long after application has ceased.
  Long-Term Cattle Manure...  
Les épandages de fumier à long terme ont accru les teneurs du sol en matière organique, en azote, en phosphore, en sel et en minéraux traces. Le rendement en paille a augmenté à toutes les doses de fumier, mais les plus fortes doses ont réduit le rendement en grains et nuit à la qualité de la culture.
A field experiment was started in 1973 to determine the safe loading capacity of soil with cattle feedlot manure. Manure was applied annually at 0, 30, 60, and 90 tonne ha-1 (wet weight) under rainfed and 0, 60, 120, and 180 tonne ha-1 under irrigated conditions. The long-term manure applications resulted in increased levels of organic matter, N, P, salt and trace minerals in soil. This increased straw yield at all manure rates, but reduced grain yields at higher rates and affected crop quality. Increased nutrient levels also increase the potential for nutrient losses and surface and groundwater contamination. The soil enrichments were long-lasting and could pose environmental threats long after application has ceased.
  Chaetoglobosins and aza...  
Or, à des doses pouvant se rencontrer dans un immeuble endommagé par les moisissures, l’exposition in vivo ou in vitro aux métabolites de certains champignons peut provoquer des changements de nature inflammatoire.
Chaetomium globosum is one of the most common species of fungi found growing on damp building materials in North America and Europe. At doses that could be experienced in a building with some mould damage, exposure to metabolites from other fungi results in inflammatory changes in vivo and in vitro. This research requires knowledge of the dominant toxins produced by fungal strains from the built environment and characterization of pure compounds for toxicity testing. We examined 25 strains of C. globosum isolated from the built environment in Canada. In varying amounts, these strains primarily produced chaetoglobosin A, C and F, chaetomugilin D, and chaetoviridin A. Spectroscopic data of the major isolated compounds are provided. Previous studies reported a number of metabolites from this species that we did not find. However, this appears to be due to misidentifications of the fungi they examined as well as problems with the analytical methods used. In addition, our data support the use of metabolite profiles for resolving the taxonomy of some economically important Chaetomium species.
  Évaluation de l'efficac...  
Les sept herbicides évalués ont été utilisés à 3 doses différentes, soient 0.5X, 1X et 2X. L'efficacité des herbicides fut évaluée en recueillant des données sur les dommages causés aux plants de ginseng (été 2006) et sur le rendement de leurs racines (au printemps 2007).
The seven herbicides evaluated were applied at three rates: 0.5X, 1X and 2X. Herbicide efficacy was evaluated by collecting data on injury caused to the ginseng plants (summer 2006) and on root yield (spring of 2007).
  Essai de contrôle des h...  
Le plan expérimental utilisé fut un bloc aléatoire complet, comportant 4 répétitions et utilisant des parcelles de 2.25m × 8m. Les sept herbicides évalués ont été utilisés à 3 doses différentes, soient 0.5×, 1× et 2×.
Field trials were conducted in 2007 at the Simcoe Research Station of the University of Guelph, in Ontario. The experimental design was a randomized complete block design with four replications. Plots were 2.25 m by 8 m. The seven herbicides evaluated were applied at three rates: 0.5×, 1× and 2×. The herbicide treatments were either pre-emergence or post-emergence to the weeds. Data were collected on injury to peas and efficacy in controlling broadleaf weeds.
  Effet d’un traitement à...  
Les concentrations de prolactine, progestérone, estradiol-17b et leptine ont été dosé es. La silymarine n’a pas augmenté les concentrations de prolactine à aucune des doses utilisées et n’a pas affecté non plus les concentrations des autres hormones mesurées (P>0.1).
An extract (silymarin) from the plant Silybum marianum (milk thistle) was shown to increase circulating concentrations of prolactin in cycling rats. A dose-response study was undertaken to determine if silymarin does have hyperprolactinemic properties in cycling swine. Forty-four weaned sows were allotted to four groups receiving 0, 1, 2 or 4 g d-1 of silymarin over a period of 8 d. Blood samples were obtained on days 1 (first day of treatment starting 24 to 48 h after the onset of the standing estrus), 2, 3, and 9. Prolactin, progesterone, estradiol-17β and leptin concentrations were determined. Silymarin did not increase prolactin concentrations at any of the doses studied, nor did it affect concentrations of the other hormones (P>0.1).
  Mise au point et impact...  
Les études de terrain ont été menées de 2004 à 2007 sur deux champs en rotation maïs-soja à Beaumont et à St-Jean-sur-Richelieu au Québec. Des sections de champs ont été traitées en post-levée soit avec des doses d'herbicide variables, soit avec une pleine dose constante.
Field studies were conducted from 2004 to 2007 on two corn and soybean crop rotations in Beaumont and St-Jean-sur-Richelieu, Quebec. Sections of fields were treated with varying in-crop herbicides, with a full constant dose. Variable doses (no herbicide, half or full doses) were based on the calculated RC value.
  Mise au point et impact...  
Les études de terrain ont été menées de 2004 à 2007 sur deux champs en rotation maïs-soja à Beaumont et à St-Jean-sur-Richelieu au Québec. Des sections de champs ont été traitées en post-levée soit avec des doses d'herbicide variables, soit avec une pleine dose constante.
Field studies were conducted from 2004 to 2007 on two corn and soybean crop rotations in Beaumont and St-Jean-sur-Richelieu, Quebec. Sections of fields were treated with varying in-crop herbicides, with a full constant dose. Variable doses (no herbicide, half or full doses) were based on the calculated RC value.
  Zebarth, Bernie, Ph.D. ...  
« Effet de différentes sources et doses de fertilisants minéraux azotés sur le lessivage potentiel des nitrates sous culture de pomme de terre. », 24e Congrès annuel de l’Association Québécoise des Spécialistes en Science du Sol (AQSSS): Vers des systèmes sol-plante sains et durables, Abbaye d’Oka, Oka, QC, Canada, June 1-3, 2010.
Cambouris, A.N. and Zebarth, B.J. (2010). "Effet de différentes sources et doses de fertilisants minéraux azotés sur le lessivage potentiel des nitrates sous culture de pomme de terre.", 24e Congrès annuel de l’Association Québécoise des Spécialistes en Science du Sol (AQSSS): Vers des systèmes sol-plante sains et durables, Abbaye d’Oka, Oka, QC, Canada, June 1-3, 2010.
  Nurse, Robert E., Ph.D....  
Soltani, N., Nurse, R.E., Van Eerd, L.L., Vyn, R.J., Shropshire, C., et Sikkema, P.H. (2010). « Weed control, environmental impact and profitability with trifluralin plus reduced doses of imazethapyr in dry bean. », Crop Protection, 29(4), p.
Soltani, N., Nurse, R.E., Van Eerd, L.L., Vyn, R.J., Shropshire, C., and Sikkema, P.H. (2010). "Weed control, environmental impact and profitability with trifluralin plus reduced doses of imazethapyr in dry bean.", Crop Protection, 29(4), pp. 364-368. doi : 10.1016/j.cropro.2009.07.011
  Panneton, Bernard, Ph.D...  
« Doses réduites d’herbicides et dynamique des populations de mauvaises herbes. », Cahier de conférence - Événement conjoint en phytoprotection, CRAAQ/SPPQ, Drummondville, QC, Canada, October 22-23, 2009.
Simard, M.-J., Panneton, B., Rouane, S., Leroux, G.D., Longchamps, L., and Légère, A. (2009). "Doses réduites d’herbicides et dynamique des populations de mauvaises herbes.", Cahier de conférence - Événement conjoint en phytoprotection, CRAAQ/SPPQ, Drummondville, QC, Canada, October 22-23, 2009.
  Weed control, environme...  
Soltani, N., Nurse, R.E., Van Eerd, L.L., Vyn, R.J., Shropshire, C., et Sikkema, P.H. (2010). « Weed control, environmental impact and profitability with trifluralin plus reduced doses of imazethapyr in dry bean. », Crop Protection, 29(4), p.
Soltani, N., Nurse, R.E., Van Eerd, L.L., Vyn, R.J., Shropshire, C., and Sikkema, P.H. (2010). "Weed control, environmental impact and profitability with trifluralin plus reduced doses of imazethapyr in dry bean.", Crop Protection, 29(4), pp. 364-368. doi : 10.1016/j.cropro.2009.07.011 Access to full text
  Toxicity of spinosad an...  
, Bélair, G., Simard, L., et Brodeur, J. (2009). « Toxicity of spinosad and Beauveria bassiana to the black cutworm, and the additivity of subletal doses. », Biocontrol Science and Technology, 19(2), p.
Gosselin, M.-E., Bélair, G., Simard, L., and Brodeur, J. (2009). "Toxicity of spinosad and Beauveria bassiana to the black cutworm, and the additivity of subletal doses.", Biocontrol Science and Technology, 19(2), pp. 201-217. doi : 10.1080/09583150802663285 Access to full text
  Mise au point et impact...  
Effectuer une analyse spatiale des mauvaises herbes présentes dans les champs de maïs et des champs de soja traités avec différents herbicides et diverses doses; valider les stratégies de gestion des mauvaises herbes au moyen de traitements herbicides localisés et de doses réduite
To conduct a spatial analysis of weed coverage in corn and soybean fields treated with various herbicides and rates and validate weed management strategies using localized and reduced rate herbicide applications
  Mise au point et impact...  
Effectuer une analyse spatiale des mauvaises herbes présentes dans les champs de maïs et des champs de soja traités avec différents herbicides et diverses doses; valider les stratégies de gestion des mauvaises herbes au moyen de traitements herbicides localisés et de doses réduite
To conduct a spatial analysis of weed coverage in corn and soybean fields treated with various herbicides and rates and validate weed management strategies using localized and reduced rate herbicide applications
  Bélair, Guy, M.Sc. - Ag...  
, Bélair, G., Simard, L., et Brodeur, J. (2009). « Toxicity of spinosad and Beauveria bassiana to the black cutworm, and the additivity of subletal doses. », Biocontrol Science and Technology, 19(2), p.
Gosselin, M.-E., Bélair, G., Simard, L., and Brodeur, J. (2009). "Toxicity of spinosad and Beauveria bassiana to the black cutworm, and the additivity of subletal doses.", Biocontrol Science and Technology, 19(2), pp. 201-217. doi : 10.1080/09583150802663285
  Vernon, Robert S., Ph.D...  
Vernon, R.S. et van Herk, W.G. (2010). « Procedes utilisant de faibles doses pour lutter contre des insectes nuisibles. », publié dedans US Patent 3 61/105.504 (2008), PCT/CA2009/001452 (2009). (Brevet)
Vernon, R.S. and van Herk, W.G. (2010). "Low dose methods for controlling inssect pests.", published in US Patent 3 61/105.504 (2008), PCT/CA2009/001452 (2009). (Patent)
  Benchaar, Chaouki, Ph.D...  
Ingestion, fermentation ruminale, résultats de croissance et caractéristiques de carcasse des agneaux recevant différentes doses de cinnamaldéhyde
Intake, ruminal fermentation, growth performance, and carcass characteristics in lambs fed different doses of cinnamaldehyde
  Toxicity of spinosad an...  
Le principal objectif de cette étude était d’évaluer la sensibilité du vers-gris noir (Agrotis ipsilon) au biopesticide spinosad et à une préparation commerciale (souche GHA) du champignon entomopathogène Beauveria bassiana. Nous avons également quantifié les effets de doses sublétales de spinosad sur certains paramètres de valeur adaptative chez l’A.
The main objective of this study was to assess the susceptibility of the black cutworm (Agrotis ipsilon) to the biopesticide spinosad and to a commercial formulation (GHA strain) of the entomopathogenic fungus, Beauveria bassiana. Secondly, we quantified the effects of sublethal doses of spinosad on a number of A. ipsilon fitness parameters, and interactions resulting from simultaneous applications of sub-lethal doses of spinosad and B. bassiana. Under laboratory conditions, A. ipsilon third instar larvae were highly susceptible to spinosad, with an estimated LC50 of 50 ppm. The entomopathogenic fungus, B. bassiana had a lower efficacy with an estimated LC50 of 7 107 spores mL-1. Topical applications of 5, 7.5 and 10 ppm of spinosad on third instar larvae reduced larval size and increased time to pupation and to emergence. However, pupal and adult weights were not significantly different between treated and control individuals. Additivity was observed from most spinosad-B. bassiana combinations tested, thus indicating compatibility between products. We concluded that spinosad is a promising tool for controlling black cutworm larvae alone or in combination with other products.
  Suitable dose of  
Nous avons irradié des graines de six obtentions de trois espèces de Roegneria avec le rayonnement gamma du 60Co, à différentes doses (50, 100, 150, 200, 250, 300 et 400 Gy). Suite à ces traitements, nous avons étudié l’énergie de germination, la vitesse de germination, la hauteur des semis, la hauteur des plants, le taux de survie des plants et la grenaison.
Seeds from six accessions of three species of Roegneria were radiated with 60Co γ-ray at different doses (50, 100, 150, 200, 250, 300 and 400 Gy). Following these treatments, germination energy, germination rate, seedling height, plant height, plant survival, and seed set were observed. Plant survival was highly correlated with seedling height (R 2 > 0.91, P < 0.01) and seed set (R 2 > 0.82, P < 0.01). The semi-lethal dose of each accession, calculated using a ‘Multi-target single-hit’ model, ranged from 60 to 173 Gy. The most suitable absorbed doses for each accession were deduced from these data. The suitable doses for Roegneria kamoji, Roegneria ciliaris and Roegneria japonensis were 65–100 Gy, 63–150 Gy and 80–170 Gy, respectively. According to the range of suitable doses, R. kamoji (Pr87-88-353) was the most sensitive to radiation, and R. japonensis (88-89-267) was the most resistant to radiation. Suitable doses of R. ciliaris were close to that of R. kamoji (ZY1007). This research provides preliminary guidelines for radiation induced mutagenesis in Roegneria.
  Identification of diffe...  
Bien qu’ils fournissent des données précieuses, les modèles in vitro du développement des adipocytes nécessitent souvent l’emploi de fortes doses d’hormones et de facteurs de croissance, ce qui peut influer sur l’expression des gènes et le processus de différenciation adipocytaire.
Although they provide valuable information, in vitro models of adipocyte development often require high doses of hormones and growth factors, which may influence gene expression and adipocyte differentiation patterns. To overcome these problems, a novel in vivo model of adipose tissue development was used to characterize genes involved in adipogenesis. The suppression subtractive hybridization technique was used to identify genes showing differential expression between the adipose tissue of a day 90 gestating sow, which is enriched in adipocytes, and day 90 fetal adipose tissue, which is enriched in preadipocytes. A total of 149 expressed sequence tags corresponding to identified genes and tentative consensus sequences emerged. Thirty-seven clones matched expressed sequence tags or genomic DNA sequences and six novel sequences were also identified. Adipogenesis-related genes were identified, many of which have never been reported to be expressed in mammalian adipose tissue, and may play a role in regulation of adipose tissue differentiation. Validation of differentially expressed genes was confirmed for perilipin, monocyte to macrophage differentiation-associated, myocilin, paraoxonase 3, stearoyl-CoA desaturase, angiotensinogen and adiponectin genes using real-time RT-PCR.
  Oral and Rectal Adminis...  
Le bactériophage a été inoculé à des bouvillons chez lesquels nous avons ensuite surveillé l’excrétion fécale de bactéries E. coli O157:H7 résistantes à l’acide nalidixique (NalR) pendant 83 jours après l’inoculation par voie orale (ORL; 3,3 × 1011 UFP), rectale (REC; 1,5 × 1011 UFP), et orale et rectale (O+R; 4.8 × 1011 UFP); nous avions aussi un groupe de bouvillons non inoculés (TEM; témoins). La solution inoculée consistait en un cocktail de quatre souches de bactériophages spécifiques de l’antigène O157, en doses multiples.
This study compared oral and rectal administration of O157-specific bacteriophages for mitigating the fecal shedding of Escherichia coli O157 by experimentally inoculated steers. Fecal shedding of nalidixic acid-resistant (NalR) E. coli O157:H7 was monitored over 83 days after oral (ORL; 3.3 × 1011 PFU), rectal (REC; 1.5 × 1011 PFU), both oral and rectal (O+R; 4.8 × 1011 PFU), or no (CON; control) treatment with a four-strain O157-specific bacteriophage cocktail in multiple doses. Bacteriophages were enumerated by plaque assay, and NalR E. coli O157:H7 by direct plating on sorbitol MacConkey agar supplemented with cefixime, potassium tellurite, and nalidixic acid. Orally treated steers produced the fewest NalR E. coli O157:H7 culture-positive samples (P < 0.06) compared with REC and O+R steers, but this number was only nominally lower (P = 0.26) than that for the CON steers. The overall mean shedding level (log CFU per gram of feces) was higher for REC steers (P < 0.10) than for steers of the other treatment groups. Despite the shedding of higher mean bacteriophage levels (log PFU per gram of feces) by ORL and O+R than by CON and REC steers, there was no difference (P > 0.05) in the number of E. coli O157-positive samples among treatments. Bacteriophage was isolated from CON steers, indicating that these steers acquired the bacteriophage from the environment and shed the phage at a level similar to that of REC steers (P = 0.39). Continuous bacteriophage therapy may be an efficacious method for mitigating shedding of E. coli O157:H7 in cattle, providing that the host bacterium does not develop resistance. This therapy may be especially advantageous if nontreated cattle can acquire this biocontrol agent from the feedlot environment.
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