kcl – Traduction – Dictionnaire Keybot

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The reactions were performed in a final volume of 100 ml and contained 10 ng of template DNA, two oligonucleotide primers (0.1 mM), 200 mM of each dNTP, 10 mM Tris-HCl, 50 mM KCl, 1.5 mM MgCl2, 0.1% (w/v) gelatin and 2U of Taq DNA polymerase (Perkin Elmer, U. S. A).
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All PCR reactions were carried out in a GeneAmp PCR system 9600 (Perkin Elmer, U. S. A.). The reactions were performed in a final volume of 100 ml and contained 10 ng of template DNA, two oligonucleotide primers (0.1 mM), 200 mM of each dNTP, 10 mM Tris-HCl, 50 mM KCl, 1.5mM MgCl2, 0.1% (w/v) gelatin and 2U of Taq DNA polymerase (Perkin Elmer, U. S. A). The PCR reaction consisted of 30 cycles of denaturation (94°C, 1 min), annealing (50°C, 1 min), and extension (72°C, 2 min).
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An aliquot of 2 µl of this suspension was added to 23 µl of PCR mixture containing 50 mM KCl, 20 mM Tris-HCl (pH 8.4), 2.5 mM MgCl2, 0.2 mM of each deoxynucleoside triphosphate (dATP, dUTP, dGTP, and dCTP) (Invitrogen Inc., Carlsbad, CA), various concentrations of the respective primers (Table 2), and 1.0 unit of Platinum Taq DNA polymerase (Invitrogen Inc., Carlsbad, CA).
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All PCR assays were performed directly from bacterial suspensions obtained after the rapid DNA extraction method. An aliquot of 2 µl of this suspension was added to 23 µl of PCR mixture containing 50 mM KCl, 20 mM Tris-HCl (pH 8.4), 2.5 mM MgCl2, 0.2 mM of each deoxynucleoside triphosphate(dATP, dUTP, dGTP, and dCTP) (Invitrogen Inc., Carlsbad, CA), various concentrations of the respective primers (Table 2), and 1.0 unit of Platinum Taq DNA polymerase (Invitrogen Inc., Carlsbad, CA).