microgel – -Translation – Keybot Dictionary

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Show textShow cached sourceOpen source URL	The radical polymerisation takes place at 70°C to obtain a microgel. The reaction starts at 60 nm...
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Show textShow cached sourceOpen source URL	Visvardis, E.E., Haveles, K.S., Pataryas, T.A.. , Mararitis, L.H., Sophianopoulou, V., Sideris, E.G., 2000 Environ Mol Mutagen 36: 32-39 Diversity of peripheral blood mononuclear cells as revealed by a novel multiple microgel Acomet assay@
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Show textShow cached sourceOpen source URL	Tomohiro Maeda, AKASAKIYusuke, YAMAMOTOKazuya, T. Aoyagi : “timuli-Responsive Coacervate Induced in Binary Functionalized Poly(N-isopropylacrylamide) Aqueous System and Novel Method for Preparing Semi-IPN Microgel Using the Coacervate” Langmuir 25[16] (2009) 9510-9517
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Intact unfixed spermatozoa are immersed in an inert agarose microgel on a pretreated slide. An initial acid treatment denatures DNA in those sperm cells with fragmented DNA. Following this, the lysing solution removes most of the nuclear proteins, and in the absence of massive DNA breakage produces nucleoids with large halos of spreading DNA loops, emerging from a central core.

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Des spermatozoïdes intacts non fixés (frais, congelés/décongelés, dilués ou non) sont immergés dans un microgel agarose inerte sur une lame prétraitée. Un traitement initial à l’acide dénature l’ADN des cellules spermatiques avec un ADN fragmenté. La solution de lyse supprime ensuite la plupart des protéines nucléaires et, en l’absence de rupture massive d’ADN, produit des nucléotides avec de grands halos de boucles d’ADN, émergeant d’un noyau central. En revanche, les spermatozoïdes avec un ADN fragmenté ne développent pas un halo de dispersion ou bien celui-ci est minime.