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The downfall of using only four functions occurs when the overhead of selecting Up/Down and CS during each adjustment slows operation down too much for the application at hand. Thus, two additional functions are included that demonstrate in/decrementing the chip (five intervals each way) with a single selection both the direction and chip select inputs.
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In addition to the routines described above, three other routines reside in the software to generate timing. They are called wait16us, wt and wait3sec. These function provide a fixed 1.6µs delay, a variable delay that is selected by the values stored in R5, R6, and R7, and a fixed 3-second delay that is used in the main program.
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One library is prepared with an oligonucleotide sequence included that when sequenced can help provide quality control for the library
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Multiple libraries were prepared each of which can be distinguished in the sequencing result through a molecular barcode or other indicator. Each library may be made from the same or different samples.