les constructions ont – English Translation – Keybot Dictionary
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www.vekaslide.md
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C’est une silhouette très spécifique de tours et de grands édifices publics (tels que la cathédrale, le beffroi et les églises) qui domine la ville. Pour la plupart,
les constructions ont
conservé les parcelles de terre originales.
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pcb-bg.com
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An area of continuous settlement, the Historic Centre of Brugge has retained the original pattern of streets and places, canals, and open spaces. A very specific skyline of towers and taller civic buildings (such as the cathedral, the belfry and the churches) dominates the city. For the most part, buildings have retained the original parcels of land. The transformations that have taken place over time respect the functional changes in the town, and have become part of its historic authenticity, in a parallel way to other historic cities such as Siena in Italy.
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whc.unesco.org
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Le site reflète la tradition architecturale spécifique de cette région, et notamment la manière dont
les constructions ont
évolué, au fil des siècles, grâce à un entretien régulier, réalisé selon des techniques traditionnelles.
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The pyramidal tomb is constructed of mud bricks faced with mud plaster. Gnarled scaffolding timbers project out from the face of the tomb and allow easy access for replastering. On the east side is a winding external stair leading to the summit. The forest of scaffolding timbers and the sculpted lines of the building, which have developed over centuries of replastering, combine to create a unique architectural piece.
csc.lexum.org
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Il semble que, dans tous les cas, les motels ont été bâtis par M. Llewellyn et, après l’achèvement, le titre de propriété a été cédé à Wandlyn Motels Ltd. Dans un seul cas, le terrain et
les constructions ont
été cédés à Kennedy Rentals Limited, mais ce n’est qu’après les événements qui ont donné lieu à la présente action qu’on a constaté ce fait.
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Charles H. Llewellyn was a contractor and builder building private homes, apartment houses, schools and other types of buildings. During the 1950’s, he began to build motels in New Brunswick. These motels were known as the Wandlyn Motels and up to 1963 he had insured them through various insurance agencies. The motels in each case seem to have been built by Mr. Llewellyn and thereafter the title in them transferred to the Wandlyn Motels Ltd. In one case, the owner of the land and buildings became the Kennedy Rentals Limited, but this circumstance was only discovered after the events with which this action is concerned.
scc.lexum.org
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Il semble que, dans tous les cas, les motels ont été bâtis par M. Llewellyn et, après l’achèvement, le titre de propriété a été cédé à Wandlyn Motels Ltd. Dans un seul cas, le terrain et
les constructions ont
été cédés à Kennedy Rentals Limited, mais ce n’est qu’après les événements qui ont donné lieu à la présente action qu’on a constaté ce fait.
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Charles H. Llewellyn was a contractor and builder building private homes, apartment houses, schools and other types of buildings. During the 1950’s, he began to build motels in New Brunswick. These motels were known as the Wandlyn Motels and up to 1963 he had insured them through various insurance agencies. The motels in each case seem to have been built by Mr. Llewellyn and thereafter the title in them transferred to the Wandlyn Motels Ltd. In one case, the owner of the land and buildings became the Kennedy Rentals Limited, but this circumstance was only discovered after the events with which this action is concerned.
nice-solarenergy.com
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Nous avons eu plusieurs variétés de neige tout au long de l’évènement, nous avons tout vu de 17°C à -10°C et même un peu de pluie, mais
les constructions ont
pu être faites sans problèmes et j’ai vraiment pris plaisir à travailler avec PRINOTH » a constaté le shaper de la FIS Nick Roma lors de la rencontre avec PRINOTH le 24 Janvier 2015.
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“We started here on January the 2nd with a great fleet of PRINOTH machines to build the world championship course. We had a variety of snow conditions throughout the way, we have seen everything from +17°C to -10°C and a little bit of rain, but the construction has gone very well and I really enjoyed working with PRINOTH,” reflected FIS Builder Nick Roma during the PRINOTH get-together on the 24th of January 2015. On that day, PRINOTH organized a special event for more than 50 clients, driver and partner. Starting with a day of skiing on the Kreischberg Mountain, exclusive grooming demos and finishing up with a cozy get-together in the “Österreich Haus” and private lounge with a direct view on the Big Air Finals.
www5.agr.gc.ca
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Chaque fragment a été cloné en phase avec un gène d’intérêt, et
les constructions ont
ensuite été introduites chez Nicotiana benthamiana par co-transformation avec Agrobacterium, ce qui permet l’expression transitoire des protéines hybrides.
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We have developed a BiFC technique to test the interaction between two proteins in vivo. This is accomplished by splitting a yellow fluorescent protein (YFP) into two non-overlapping fragments. Each fragment is cloned in-frame to a gene of interest. These constructs can then be co-transformed into Nicotiana benthamiana via Agrobacterium mediated transformation, allowing the transit expression of fusion proteins. The reconstitution of YFP signal only occurs when the inquest proteins interact. To test and validate the protein-protein interactions, BiFC can be used together with yeast two hybrid (Y2H) assay. This may detect indirect interactions which can be overlooked in the Y2H. Gateway technology is a universal platform that enables researchers to shuttle the gene of interest (GOI) into as many expression and functional analysis systems as possible. Both the orientation and reading frame can be maintained without using restriction enzymes or ligation to make expression-ready clones. As a result, one can eliminate all the re-sequencing steps to ensure consistent results throughout the experiments. We have created a series of Gateway compatible BiFC and Y2H vectors which provide researchers with easy-to-use tools to perform both BiFC and Y2H assays. Here, we demonstrate the ease of using our BiFC system to test protein-protein interactions in N. benthamiana plants.
www.agr.ca
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Chaque fragment a été cloné en phase avec un gène d’intérêt, et
les constructions ont
ensuite été introduites chez Nicotiana benthamiana par co-transformation avec Agrobacterium, ce qui permet l’expression transitoire des protéines hybrides.
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agr.ca
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We have developed a BiFC technique to test the interaction between two proteins in vivo. This is accomplished by splitting a yellow fluorescent protein (YFP) into two non-overlapping fragments. Each fragment is cloned in-frame to a gene of interest. These constructs can then be co-transformed into Nicotiana benthamiana via Agrobacterium mediated transformation, allowing the transit expression of fusion proteins. The reconstitution of YFP signal only occurs when the inquest proteins interact. To test and validate the protein-protein interactions, BiFC can be used together with yeast two hybrid (Y2H) assay. This may detect indirect interactions which can be overlooked in the Y2H. Gateway technology is a universal platform that enables researchers to shuttle the gene of interest (GOI) into as many expression and functional analysis systems as possible. Both the orientation and reading frame can be maintained without using restriction enzymes or ligation to make expression-ready clones. As a result, one can eliminate all the re-sequencing steps to ensure consistent results throughout the experiments. We have created a series of Gateway compatible BiFC and Y2H vectors which provide researchers with easy-to-use tools to perform both BiFC and Y2H assays. Here, we demonstrate the ease of using our BiFC system to test protein-protein interactions in N. benthamiana plants.
www.listeriosis-listeriose.investigation-enquete.gc.ca
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Chaque fragment a été cloné en phase avec un gène d’intérêt, et
les constructions ont
ensuite été introduites chez Nicotiana benthamiana par co-transformation avec Agrobacterium, ce qui permet l’expression transitoire des protéines hybrides.
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listeriosis-listeriose.investigation-enquete.gc.ca
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We have developed a BiFC technique to test the interaction between two proteins in vivo. This is accomplished by splitting a yellow fluorescent protein (YFP) into two non-overlapping fragments. Each fragment is cloned in-frame to a gene of interest. These constructs can then be co-transformed into Nicotiana benthamiana via Agrobacterium mediated transformation, allowing the transit expression of fusion proteins. The reconstitution of YFP signal only occurs when the inquest proteins interact. To test and validate the protein-protein interactions, BiFC can be used together with yeast two hybrid (Y2H) assay. This may detect indirect interactions which can be overlooked in the Y2H. Gateway technology is a universal platform that enables researchers to shuttle the gene of interest (GOI) into as many expression and functional analysis systems as possible. Both the orientation and reading frame can be maintained without using restriction enzymes or ligation to make expression-ready clones. As a result, one can eliminate all the re-sequencing steps to ensure consistent results throughout the experiments. We have created a series of Gateway compatible BiFC and Y2H vectors which provide researchers with easy-to-use tools to perform both BiFC and Y2H assays. Here, we demonstrate the ease of using our BiFC system to test protein-protein interactions in N. benthamiana plants.