rpos – English Translation – Keybot Dictionary

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  3 Hits www.novell.com  
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Learn how to accelerate your consolidation and virtualization initiatives and improve recovery time objectives (RTOs) and recovery point objectives (RPOs) without expensive duplication.
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  8 Hits www.fundit.fr  
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The proposal should demonstrate how the Research Integrity Promotion Plans will contribute to the promotion of research integrity, fostering a culture of open science and open innovation. The work will also propose methods for monitoring the implementation of such integrity plans in RPOs and RFOs.
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The proposed actions will closely collaborate with and make use of the results form relevant EU funded research projects under the SWAFs programme (mainly PRINTEGER, ENERI, DEFORM and projects funded via SwafS-16-2016, SwafS-21-2017, SwafS-27-2017). The currently available results of these projects are accessible through the websites already listed (see previous footnote). Any IT communication infrastructure envisioned should use the existing EU communication tool SINAPSE[6]
  agritrade.cta.int  
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9. Agronomes et vétérinaires sans frontières (AVF)/Vétérinaires sans frontières Belgique (VSFB)/Association des organisations professionnelles paysannes (AOPP)/Initiatives, conseils, développement (ICD), « Local milk sector in West Africa: Role of RPOs, small and medium farmers in the full growth of its potential », rapport d’un atelier tenu à Bamako du 15 au 17 septembre 2010, publié en 2011
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9. Agronomes et vétérinaires sans frontières (AVF)/Vétérinaires sans frontières Belgique (VSFB)/Association des organisations professionnelles paysannes (AOPP)/Initiatives, conseils, développement (ICD), ‘Local milk sector in West Africa: Role of RPOs, small and medium farmers in the full growth of its potential’, on a workshop held in Bamako on 15–17 September 2010, posted 2011
  www.listeriosis-listeriose.investigation-enquete.gc.ca  
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Un essai quantitatif comparatif par la technique RT-PCR en temps réel en deux étapes a été utilisé pour évaluer l'expression des gènes codant la sous-unité A des toxines 1 et 2 de Shiga (stx1A et stx2A), l’intimine (eaeA), la flagelline (fliC), σS - le facteur sigma du stress général (rpoS) et la superoxyde dismutase à fer (sodB) chez E. coli O157:H7.
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Foodborne outbreaks attributed to the contamination of fresh produce with Escherichia coli O157:H7 are a growing concern. In particular, leafy-green vegetables, including lettuce and spinach, are susceptible to contamination by irrigation water, manure, and food processing and storage practices. The survival of E. coli O157:H7 and natural microflora on Romaine lettuce stored at 4 °C and 15 °C over a 9-day period was evaluated by plate counts. A two-step reverse-transcription comparative quantitative real-time PCR assay was employed to evaluate expression of genes coding for the A subunit of Shiga-toxin 1 and 2 (stx1A and stx2A), intimin (eaeA), flagellin (fliC), σS - general stress sigma factor (rpoS) and iron superoxide dismutase (sodB) in E. coli O157:H7. Results indicate that reducing the storage temperature from 15 °C to 4 °C significantly (P < 0.05) reduced the growth of Escherichia coli O157:H7 on Romaine lettuce, however, viable populations remained after the end of both storage periods. At end of the storage period, a 0.430 and 0.180 log decrease in E. coli O157:H7 was observed at 4 °C and 15 °C, respectively. Under both storage temperatures, total aerobic plate counts increased over the duration of the experiment. An increase in E. coli O157:H7 fold expression was observed with stx2A. Although stx1A exhibited upregulation for all storage conditions, variable gene expression was observed throughout the storage period. In addition, fliC was up-regulated during storage at 15 °C, while transcription at 4 °C storage changed only slightly. Expression of eaeA was variable at 15 °C with a tendency towards down-regulation, however, this gene was slightly up-regulated when stored at 4 °C. Slight upregulation of rpoS and sodB was also observed at 4 °C. In conclusion, our results suggest E. coli O157:H7 may become more virulent with prolonged storage of Romaine lettuce, particularly when stored at refrigerated temperatures.
  www.agr.ca  
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Un essai quantitatif comparatif par la technique RT-PCR en temps réel en deux étapes a été utilisé pour évaluer l'expression des gènes codant la sous-unité A des toxines 1 et 2 de Shiga (stx1A et stx2A), l’intimine (eaeA), la flagelline (fliC), σS - le facteur sigma du stress général (rpoS) et la superoxyde dismutase à fer (sodB) chez E. coli O157:H7.
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Foodborne outbreaks attributed to the contamination of fresh produce with Escherichia coli O157:H7 are a growing concern. In particular, leafy-green vegetables, including lettuce and spinach, are susceptible to contamination by irrigation water, manure, and food processing and storage practices. The survival of E. coli O157:H7 and natural microflora on Romaine lettuce stored at 4 °C and 15 °C over a 9-day period was evaluated by plate counts. A two-step reverse-transcription comparative quantitative real-time PCR assay was employed to evaluate expression of genes coding for the A subunit of Shiga-toxin 1 and 2 (stx1A and stx2A), intimin (eaeA), flagellin (fliC), σS - general stress sigma factor (rpoS) and iron superoxide dismutase (sodB) in E. coli O157:H7. Results indicate that reducing the storage temperature from 15 °C to 4 °C significantly (P < 0.05) reduced the growth of Escherichia coli O157:H7 on Romaine lettuce, however, viable populations remained after the end of both storage periods. At end of the storage period, a 0.430 and 0.180 log decrease in E. coli O157:H7 was observed at 4 °C and 15 °C, respectively. Under both storage temperatures, total aerobic plate counts increased over the duration of the experiment. An increase in E. coli O157:H7 fold expression was observed with stx2A. Although stx1A exhibited upregulation for all storage conditions, variable gene expression was observed throughout the storage period. In addition, fliC was up-regulated during storage at 15 °C, while transcription at 4 °C storage changed only slightly. Expression of eaeA was variable at 15 °C with a tendency towards down-regulation, however, this gene was slightly up-regulated when stored at 4 °C. Slight upregulation of rpoS and sodB was also observed at 4 °C. In conclusion, our results suggest E. coli O157:H7 may become more virulent with prolonged storage of Romaine lettuce, particularly when stored at refrigerated temperatures.
  www5.agr.gc.ca  
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Un essai quantitatif comparatif par la technique RT-PCR en temps réel en deux étapes a été utilisé pour évaluer l'expression des gènes codant la sous-unité A des toxines 1 et 2 de Shiga (stx1A et stx2A), l’intimine (eaeA), la flagelline (fliC), σS - le facteur sigma du stress général (rpoS) et la superoxyde dismutase à fer (sodB) chez E. coli O157:H7.
Compare text pagesCompare HTM pagesOpen source URLOpen target URLDefine www5.agr.gc.ca as primary domain
Foodborne outbreaks attributed to the contamination of fresh produce with Escherichia coli O157:H7 are a growing concern. In particular, leafy-green vegetables, including lettuce and spinach, are susceptible to contamination by irrigation water, manure, and food processing and storage practices. The survival of E. coli O157:H7 and natural microflora on Romaine lettuce stored at 4 °C and 15 °C over a 9-day period was evaluated by plate counts. A two-step reverse-transcription comparative quantitative real-time PCR assay was employed to evaluate expression of genes coding for the A subunit of Shiga-toxin 1 and 2 (stx1A and stx2A), intimin (eaeA), flagellin (fliC), σS - general stress sigma factor (rpoS) and iron superoxide dismutase (sodB) in E. coli O157:H7. Results indicate that reducing the storage temperature from 15 °C to 4 °C significantly (P < 0.05) reduced the growth of Escherichia coli O157:H7 on Romaine lettuce, however, viable populations remained after the end of both storage periods. At end of the storage period, a 0.430 and 0.180 log decrease in E. coli O157:H7 was observed at 4 °C and 15 °C, respectively. Under both storage temperatures, total aerobic plate counts increased over the duration of the experiment. An increase in E. coli O157:H7 fold expression was observed with stx2A. Although stx1A exhibited upregulation for all storage conditions, variable gene expression was observed throughout the storage period. In addition, fliC was up-regulated during storage at 15 °C, while transcription at 4 °C storage changed only slightly. Expression of eaeA was variable at 15 °C with a tendency towards down-regulation, however, this gene was slightly up-regulated when stored at 4 °C. Slight upregulation of rpoS and sodB was also observed at 4 °C. In conclusion, our results suggest E. coli O157:H7 may become more virulent with prolonged storage of Romaine lettuce, particularly when stored at refrigerated temperatures.