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In contrast LC based separation methods might underestimate the total toxin levels as those methods resolve each compound as a
single parameter
and are usually only developed for the parent mycotoxins.
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There are two ways to detect and quantify modified mycotoxins: A “direct” approach that measures the whole modified compound, and an “indirect” approach that measures the parent compound after chemical or enzymatic treatments that lead to the cleavage of modified mycotoxins, mainly by hydrolysis. Among the advantages of the indirect method are that reference materials for modified mycotoxins are not needed for correct quantification, and that all modified forms are included in the final result. The main disadvantages are that the efficiency of the hydrolysis process cannot be verified easily, and that there is no access to the quantities of the different forms of a toxin. Therefore, it is important to develop direct methods to obtain further insight into the occurrence of modified mycotoxins. All chromatographic technologies for parent mycotoxins are also potentially suitable for their modified forms as long as they are soluble and directly availablefor analysis.
