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Waarschijnlijk komt HPV DNA ook in de lymfklier voor in immunocompetente cellen, zoals macrofagen. Het aantonen van RNA, hetgeen zeer gevoelig is voor degradatie, sluit aantonen van dode tumorcellen uit.
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Carcinoma of the uterine cervix is one of the most frequent forms of cancer in women. The association between cervical cancer and the human papillomavirus (HPV) is undisputable. This association presents the possibility to use the presence of HPV as a diagnostic/prognostic indicator. Earlier investigations of the presence of HPV DNA in lymph nodes from women with early stage cervical cancer have shown that there is no prognostic value. Most likely HPV DNA can also be present in lymph nodes internalized in immunocompetent cells, such as macrophages. Detection of RNA, being extremely sensitive to degradation, excludes the presence of dead tumour cells. Follow-up of patients should give an indication of the prognostic value of RNA detection.
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Waarschijnlijk komt HPV DNA ook in de lymfklier voor in immunocompetente cellen, zoals macrofagen. Het aantonen van RNA, hetgeen zeer gevoelig is voor degradatie, sluit aantonen van dode tumorcellen uit.
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Carcinoma of the uterine cervix is one of the most frequent forms of cancer in women. The association between cervical cancer and the human papillomavirus (HPV) is undisputable. This association presents the possibility to use the presence of HPV as a diagnostic/prognostic indicator. Earlier investigations of the presence of HPV DNA in lymph nodes from women with early stage cervical cancer have shown that there is no prognostic value. Most likely HPV DNA can also be present in lymph nodes internalized ini mmuno competent cells, such as macrophages. Detection of RNA, being extremely sensitive to degradation, excludes the presence of deed tumors cells. Follow-up of patients should give an indication of the prognostic value of RNA detection.
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Doelstellingen: Ontwikkeling van een fysiologisch (bioenergetisch) model voor de lange termijn opname en accumulatie van prioritaire microcontaminanten door de zeehond. Evaluatie van het model door vergelijking van voorspellingen met analyseresultaten van microcontaminanten in bloed en biopsie (levende dieren) en andere weefsels (dode dieren).
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Aims: Development of a physiologically based pharmacokinetic model for long-term uptake and accumulation of microcontaminants by seals. Model evaluation by comparison of predicted values with observed results of microcontaminants in blood and biopsy (living animals) and other tissues (dead animals). Determination of the condition of seals by use of general condition-indices and more specific indications for homeostasis and stress by analysis of bloodsamples with special attention for endocrine effects and immunity. Making connections between exposure, accumulation and effects. By comparison with results from analysis of animals from different areas and by use of multivariate statistical methods we will check whether it is possible to find the cause of the effects.